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Improved diagnostic and control methodologies for livestock diseases in Lao PDR and Yunnan Province, PRC

Project ID

AS1/1994/038

Project Country

Commissioned Organisation

CSIRO Australian Animal Health Laboratory, Australia

Project Leader

Dr Laurence Gleeson

Email

Laurence.Gleeson@csiro.au

Phone: 

(03) 5227-5038

Fax: 

(03) 5227-5555

Collaborating Institutions

Department of Livestock and Fisheries, Laos
Yunnan Veterinary General Station, China

Project Budget

$974,296.00

Start Date

01/01/1997

Finish Date

31/12/1999

Extension Start Date

01/01/2002

Extension Finish Date

30/06/2003

ACIAR Research Program Manager

Dr John Copland

Overview Objectives

This study aimed to improve staff skills in disease surveillance, diagnosis, epidemiology and laboratory technology. The DLF would then be able to set up immunisation programs, monitor their effectiveness, assess the significance of particular diseases, and develop animal health information systems.

Project Background and Objectives

Lao PDR is a natural corridor through which livestock travel between markets in southern China, Vietnam, Cambodia and Thailand. Most of the country's 4.5 million people are involved in subsistence farming; livestock raising is their main source of cash income and contributes 40% of Lao PDR's agricultural gross domestic product (GDP).
The Department of Livestock and Fisheries (DLF) in Lao PDR had set up a four-tiered network of veterinary workers - at village, district, province and national levels - but the network was under-resourced, lacking skills and equipment. As a result, no detailed information was available about the frequent outbreaks of disease in buffalo, cattle, chickens and pigs, and there were no effective national control and surveillance programs. This reduced the nation's prosperity and also raised international concern about through-traffic of stock and animal products.
This project, the first ACIAR Animal Science project in Lao PDR, had the major involvement of an Australian volunteer (Australian Volunteers International, AVI) based at Vientiane. It focused on foot-and-mouth disease virus (FMDV) in ruminants and pigs, and classical swine fever virus (CSFV). Both these highly contagious diseases spread readily and caused concern in Yunnan Province, China, where the established veterinary network lacked the techniques to detect specific viruses and their antibodies.

Progress Reports (Year 1, 2, 3 etc)

Year 1

Implementation of CSF preventative and control strategies in village production systems combined with longitudinal studies to measure their effectiveness and sustainability
(a) Swine production and health survey in selected villages of Bolikhamxay province
Baseline surveys were conducted in May 2002 in 8 villages, 4 villages in each of 2 districts, namely Bolikhan and Pakading, to gather information on village demography, pig management, production and health.
On a monthly basis pig production and health data are collected from each village
As yet no major losses of stock have occurred as a result of CSF or other infectious/contagious agents during the course of the survey. However, a laboratory confirmed outbreak of CSF occurred in Nampa village in Bolikhan district approximately 2 months prior to the commencement of the survey, killing more than 100 pigs.
The average birthing rates to date in Bolikhan and Pakading districts are 6 and 5.7 piglets/sow respectively
Approximately 58% of total pigs outgoing from farm (deaths, sales and home consumption) are piglets in the age group 0 '€" 3 months. The break down of information regarding out-of-village sales is on going.
The price per kilo live weight offered to farmers was 6000 kip. Considering the majority of sales are in the age bracket 0 '€" 3 months, farmers are receiving only a few dollars for their stock. There are a number of reasons why this could be the case, (i) market driven (consumers prefer young pig meat), (ii) fear of disease, (iii) not enough feed to hold piglets longer or (iv) a combination of the first 3 points.
Approximately 9% of the production system output is lost to the farmers through death of stock, of this 9%, approximately 79% of deaths occur in stock 0 '€" 3 months of age. Further data analysis is necessary.
Training has been carried out to allow this survey to be conducted.

(b) Maternal transfer of local CSF vaccine derived antibodies in controlled lab based experiments and under less controlled village-based experiments
This study is in progress. 4 laboratory-based pigs have been mated and are pregnant. The first piglets are due to be born in late March 2003. Flooding of the laboratory pens in July '€" September 2002 resulted in an unavoidable delay in commencing this experiment. It is due to be completed within the next three months
Contracts are currently being negotiated with farmers in Nampa village in Bolikhan district to carry out maternal antibody transfer experiments in 4 pregnant sows in the villages. It is expected that piglets will be born in April 2003.

Complete investigations of the epidemiology of recent FMD outbreaks in the north of the Lao PDR
Structured retrospective epidemiological investigations of FMD outbreaks in Luang Namtha and Xieng Khouang provinces of the Lao PDR were carried out in September and October 2002. Outbreaks of FMD had not been previously observed in either of these two provinces. In both locations type O caused the outbreaks that occurred in naïve herds, as farmers in outbreak areas had not previously recognised FMD in their stock. Outbreaks were found to be the result of the introduction of infected stock (Xieng Khouang) and possibly through the importation of contaminated meat (Luang Namtha). The principle mode of spread of the virus once established in both provinces was the within and between village contact of animals and the localised movement and trade of animals. The latter seemed to be exacerbated by the common practice of farmers selling their animals when they become sick and '€œmiddleman'€ traders taking advantage of farmers'€™ willingness to sell their animals at a lower price. The pattern of sale and the movement of animals for this purpose were quite varied between Luang Namtha and Xieng Khouang. The frequency of trade in each province was not determined, however this practice was far more obvious in Xieng Khouang where trade was recognised by farmers as a risk high factor (particularly Nong Het district) and more commonly transcended the bounds of local trade, with animals moving to the Vietnamese border from Paek and Kham districts. The outbreak in Luang Namtha resolved after a few months through animal movement restriction, vaccination and the remoteness and difficulty of the terrain. The outbreak in Xieng Khouang has not yet been resolved (as of Jan 2003 outbreaks are still occurring).
Further investigations and surveillance in Xieng Khouang are planned for May 2003.
During the investigation of FMD in Luang Namtha, it became apparent that a substantial number of buffaloes are transported down the Mekong River from Xoploy in Long district (LN province) to Huay Xay in Bokeo province. These animals are not traded legally and pose a significant threat to Lao, Myanmar and Thailand buffalo, cattle and pig populations. It is planned to carry out an investigation to determine the actual number of animals being traded each month on this route and carry out serological surveillance.

Evaluation of modified serological tests for FMD
Initial tests have been completed. A problem with the rabbit and guinea pig antibodies used in the ELISA were suspected. This has been confirmed and it is expected that this technology will be successfully transferred by June 2003.

Conduct training and refresher courses for district animal health staff involved in the field investigations of CSF vaccination and refresh the diagnostic sample submission network (DSSN) where appropriate
Initial training relating to the survey being carried out in Bolikhamxay province was conducted in May 2002. This training was not a particular course as such, but rather a detailed look at/discussion of the research objectives of the Bolikhamxay field study and how we would achieve these objectives. Issues covered included: (i) research plan and expected outputs (ii) information collection and recording (iii) a detailed breakdown of the monthly survey forms was carried out so that provincial and district staff responsible for the survey fully understood what information is collected and (iv) sample collection and submission.

Year 2

Implementation of CSF preventative and control strategies in village production systems combined with longitudinal studies to measure their effectiveness and sustainability
(a) Swine production and health survey in selected villages of Bolikhamxay province
Baseline surveys were conducted in May 2002 in 8 villages, 4 villages in each of 2 districts, namely Bolikhan and Pakading, to gather information on village demography, pig management, production and health.
On a monthly basis pig production and health data are collected from each village
As yet no major losses of stock have occurred as a result of CSF or other infectious/contagious agents during the course of the survey. However, a laboratory confirmed outbreak of CSF occurred in Nampa village in Bolikhan district approximately 2 months prior to the commencement of the survey, killing more than 100 pigs.
The average birthing rates to date in Bolikhan and Pakading districts are 6 and 5.7 piglets/sow respectively
Approximately 58% of total pigs outgoing from farm (deaths, sales and home consumption) are piglets in the age group 0 '€" 3 months. The break down of information regarding out-of-village sales is on going.
The price per kilo live weight offered to farmers was 6000 kip. Considering the majority of sales are in the age bracket 0 '€" 3 months, farmers are receiving only a few dollars for their stock. There are a number of reasons why this could be the case, (i) market driven (consumers prefer young pig meat), (ii) fear of disease, (iii) not enough feed to hold piglets longer or (iv) a combination of the first 3 points.
Approximately 9% of the production system output is lost to the farmers through death of stock, of this 9%, approximately 79% of deaths occur in stock 0 '€" 3 months of age. Further data analysis is necessary.
Training has been carried out to allow this survey to be conducted.

(b) Maternal transfer of local CSF vaccine derived antibodies in controlled lab based experiments and under less controlled village-based experiments
This study is in progress. 4 laboratory-based pigs have been mated and are pregnant. The first piglets are due to be born in late March 2003. Flooding of the laboratory pens in July '€" September 2002 resulted in an unavoidable delay in commencing this experiment. It is due to be completed within the next three months
Contracts are currently being negotiated with farmers in Nampa village in Bolikhan district to carry out maternal antibody transfer experiments in 4 pregnant sows in the villages. It is expected that piglets will be born in April 2003.

Complete investigations of the epidemiology of recent FMD outbreaks in the north of the Lao PDR
Structured retrospective epidemiological investigations of FMD outbreaks in Luang Namtha and Xieng Khouang provinces of the Lao PDR were carried out in September and October 2002. Outbreaks of FMD had not been previously observed in either of these two provinces. In both locations type O caused the outbreaks that occurred in naïve herds, as farmers in outbreak areas had not previously recognised FMD in their stock. Outbreaks were found to be the result of the introduction of infected stock (Xieng Khouang) and possibly through the importation of contaminated meat (Luang Namtha). The principle mode of spread of the virus once established in both provinces was the within and between village contact of animals and the localised movement and trade of animals. The latter seemed to be exacerbated by the common practice of farmers selling their animals when they become sick and '€œmiddleman'€ traders taking advantage of farmers' willingness to sell their animals at a lower price. The pattern of sale and the movement of animals for this purpose were quite varied between Luang Namtha and Xieng Khouang. The frequency of trade in each province was not determined, however this practice was far more obvious in Xieng Khouang where trade was recognised by farmers as a risk high factor (particularly Nong Het district) and more commonly transcended the bounds of local trade, with animals moving to the Vietnamese border from Paek and Kham districts. The outbreak in Luang Namtha resolved after a few months through animal movement restriction, vaccination and the remoteness and difficulty of the terrain. The outbreak in Xieng Khouang has not yet been resolved (as of Jan 2003 outbreaks are still occurring).
Further investigations and surveillance in Xieng Khouang are planned for May 2003.
During the investigation of FMD in Luang Namtha, it became apparent that a substantial number of buffaloes are transported down the Mekong River from Xoploy in Long district (LN province) to Huay Xay in Bokeo province. These animals are not traded legally and pose a significant threat to Lao, Myanmar and Thailand buffalo, cattle and pig populations. It is planned to carry out an investigation to determine the actual number of animals being traded each month on this route and carry out serological surveillance.

Evaluation of modified serological tests for FMD
Initial tests have been completed. A problem with the rabbit and guinea pig antibodies used in the ELISA were suspected. This has been confirmed and it is expected that this technology will be successfully transferred by June 2003.

Conduct training and refresher courses for district animal health staff involved in the field investigations of CSF vaccination and refresh the diagnostic sample submission network (DSSN) where appropriate
Initial training relating to the survey being carried out in Bolikhamxay province was conducted in May 2002. This training was not a particular course as such, but rather a detailed look at/discussion of the research objectives of the Bolikhamxay field study and how we would achieve these objectives. Issues covered included: (i) research plan and expected outputs (ii) information collection and recording (iii) a detailed breakdown of the monthly survey forms was carried out so that provincial and district staff responsible for the survey fully understood what information is collected and (iv) sample collection and submission.

Year 3

Implementation of CSF preventative and control strategies in village production systems combined with longitudinal studies to measure their effectiveness and sustainability
(a) Swine production and health survey in selected villages of Bolikhamxay province
Baseline surveys were conducted in May 2002 in 8 villages, 4 villages in each of 2 districts, namely Bolikhan and Pakading, to gather information on village demography, pig management, production and health.
On a monthly basis pig production and health data are collected from each village
As yet no major losses of stock have occurred as a result of CSF or other infectious/contagious agents during the course of the survey. However, a laboratory confirmed outbreak of CSF occurred in Nampa village in Bolikhan district approximately 2 months prior to the commencement of the survey, killing more than 100 pigs.
The average birthing rates to date in Bolikhan and Pakading districts are 6 and 5.7 piglets/sow respectively
Approximately 58% of total pigs outgoing from farm (deaths, sales and home consumption) are piglets in the age group 0 '€" 3 months. The break down of information regarding out-of-village sales is on going.
The price per kilo live weight offered to farmers was 6000 kip. Considering the majority of sales are in the age bracket 0 '€" 3 months, farmers are receiving only a few dollars for their stock. There are a number of reasons why this could be the case, (i) market driven (consumers prefer young pig meat), (ii) fear of disease, (iii) not enough feed to hold piglets longer or (iv) a combination of the first 3 points.
Approximately 9% of the production system output is lost to the farmers through death of stock, of this 9%, approximately 79% of deaths occur in stock 0 '€" 3 months of age. Further data analysis is necessary.
Training has been carried out to allow this survey to be conducted.

(b) Maternal transfer of local CSF vaccine derived antibodies in controlled lab based experiments and under less controlled village-based experiments
This study is in progress. 4 laboratory-based pigs have been mated and are pregnant. The first piglets are due to be born in late March 2003. Flooding of the laboratory pens in July '€" September 2002 resulted in an unavoidable delay in commencing this experiment. It is due to be completed within the next three months
Contracts are currently being negotiated with farmers in Nampa village in Bolikhan district to carry out maternal antibody transfer experiments in 4 pregnant sows in the villages. It is expected that piglets will be born in April 2003.

Complete investigations of the epidemiology of recent FMD outbreaks in the north of the Lao PDR
Structured retrospective epidemiological investigations of FMD outbreaks in Luang Namtha and Xieng Khouang provinces of the Lao PDR were carried out in September and October 2002. Outbreaks of FMD had not been previously observed in either of these two provinces. In both locations type O caused the outbreaks that occurred in naïve herds, as farmers in outbreak areas had not previously recognised FMD in their stock. Outbreaks were found to be the result of the introduction of infected stock (Xieng Khouang) and possibly through the importation of contaminated meat (Luang Namtha). The principle mode of spread of the virus once established in both provinces was the within and between village contact of animals and the localised movement and trade of animals. The latter seemed to be exacerbated by the common practice of farmers selling their animals when they become sick and '€œmiddleman'€ traders taking advantage of farmers' willingness to sell their animals at a lower price. The pattern of sale and the movement of animals for this purpose were quite varied between Luang Namtha and Xieng Khouang. The frequency of trade in each province was not determined, however this practice was far more obvious in Xieng Khouang where trade was recognised by farmers as a risk high factor (particularly Nong Het district) and more commonly transcended the bounds of local trade, with animals moving to the Vietnamese border from Paek and Kham districts. The outbreak in Luang Namtha resolved after a few months through animal movement restriction, vaccination and the remoteness and difficulty of the terrain. The outbreak in Xieng Khouang has not yet been resolved (as of Jan 2003 outbreaks are still occurring).
Further investigations and surveillance in Xieng Khouang are planned for May 2003.
During the investigation of FMD in Luang Namtha, it became apparent that a substantial number of buffaloes are transported down the Mekong River from Xoploy in Long district (LN province) to Huay Xay in Bokeo province. These animals are not traded legally and pose a significant threat to Lao, Myanmar and Thailand buffalo, cattle and pig populations. It is planned to carry out an investigation to determine the actual number of animals being traded each month on this route and carry out serological surveillance.

Evaluation of modified serological tests for FMD
Initial tests have been completed. A problem with the rabbit and guinea pig antibodies used in the ELISA were suspected. This has been confirmed and it is expected that this technology will be successfully transferred by June 2003.

Conduct training and refresher courses for district animal health staff involved in the field investigations of CSF vaccination and refresh the diagnostic sample submission network (DSSN) where appropriate
Initial training relating to the survey being carried out in Bolikhamxay province was conducted in May 2002. This training was not a particular course as such, but rather a detailed look at/discussion of the research objectives of the Bolikhamxay field study and how we would achieve these objectives. Issues covered included: (i) research plan and expected outputs (ii) information collection and recording (iii) a detailed breakdown of the monthly survey forms was carried out so that provincial and district staff responsible for the survey fully understood what information is collected and (iv) sample collection and submission.

Year 4

Implementation of CSF preventative and control strategies in village production systems combined with longitudinal studies to measure their effectiveness and sustainability
(a) Swine production and health survey in selected villages of Bolikhamxay province
Baseline surveys were conducted in May 2002 in 8 villages, 4 villages in each of 2 districts, namely Bolikhan and Pakading, to gather information on village demography, pig management, production and health.
On a monthly basis pig production and health data are collected from each village
As yet no major losses of stock have occurred as a result of CSF or other infectious/contagious agents during the course of the survey. However, a laboratory confirmed outbreak of CSF occurred in Nampa village in Bolikhan district approximately 2 months prior to the commencement of the survey, killing more than 100 pigs.
The average birthing rates to date in Bolikhan and Pakading districts are 6 and 5.7 piglets/sow respectively
Approximately 58% of total pigs outgoing from farm (deaths, sales and home consumption) are piglets in the age group 0 '€" 3 months. The break down of information regarding out-of-village sales is on going.
The price per kilo live weight offered to farmers was 6000 kip. Considering the majority of sales are in the age bracket 0 '€" 3 months, farmers are receiving only a few dollars for their stock. There are a number of reasons why this could be the case, (i) market driven (consumers prefer young pig meat), (ii) fear of disease, (iii) not enough feed to hold piglets longer or (iv) a combination of the first 3 points.
Approximately 9% of the production system output is lost to the farmers through death of stock, of this 9%, approximately 79% of deaths occur in stock 0 '€" 3 months of age. Further data analysis is necessary.
Training has been carried out to allow this survey to be conducted.

(b) Maternal transfer of local CSF vaccine derived antibodies in controlled lab based experiments and under less controlled village-based experiments
This study is in progress. 4 laboratory-based pigs have been mated and are pregnant. The first piglets are due to be born in late March 2003. Flooding of the laboratory pens in July '€" September 2002 resulted in an unavoidable delay in commencing this experiment. It is due to be completed within the next three months
Contracts are currently being negotiated with farmers in Nampa village in Bolikhan district to carry out maternal antibody transfer experiments in 4 pregnant sows in the villages. It is expected that piglets will be born in April 2003.

Complete investigations of the epidemiology of recent FMD outbreaks in the north of the Lao PDR
Structured retrospective epidemiological investigations of FMD outbreaks in Luang Namtha and Xieng Khouang provinces of the Lao PDR were carried out in September and October 2002. Outbreaks of FMD had not been previously observed in either of these two provinces. In both locations type O caused the outbreaks that occurred in naïve herds, as farmers in outbreak areas had not previously recognised FMD in their stock. Outbreaks were found to be the result of the introduction of infected stock (Xieng Khouang) and possibly through the importation of contaminated meat (Luang Namtha). The principle mode of spread of the virus once established in both provinces was the within and between village contact of animals and the localised movement and trade of animals. The latter seemed to be exacerbated by the common practice of farmers selling their animals when they become sick and '€œmiddleman'€ traders taking advantage of farmers' willingness to sell their animals at a lower price. The pattern of sale and the movement of animals for this purpose were quite varied between Luang Namtha and Xieng Khouang. The frequency of trade in each province was not determined, however this practice was far more obvious in Xieng Khouang where trade was recognised by farmers as a risk high factor (particularly Nong Het district) and more commonly transcended the bounds of local trade, with animals moving to the Vietnamese border from Paek and Kham districts. The outbreak in Luang Namtha resolved after a few months through animal movement restriction, vaccination and the remoteness and difficulty of the terrain. The outbreak in Xieng Khouang has not yet been resolved (as of Jan 2003 outbreaks are still occurring).
Further investigations and surveillance in Xieng Khouang are planned for May 2003.
During the investigation of FMD in Luang Namtha, it became apparent that a substantial number of buffaloes are transported down the Mekong River from Xoploy in Long district (LN province) to Huay Xay in Bokeo province. These animals are not traded legally and pose a significant threat to Lao, Myanmar and Thailand buffalo, cattle and pig populations. It is planned to carry out an investigation to determine the actual number of animals being traded each month on this route and carry out serological surveillance.

Evaluation of modified serological tests for FMD
Initial tests have been completed. A problem with the rabbit and guinea pig antibodies used in the ELISA were suspected. This has been confirmed and it is expected that this technology will be successfully transferred by June 2003.

Conduct training and refresher courses for district animal health staff involved in the field investigations of CSF vaccination and refresh the diagnostic sample submission network (DSSN) where appropriate
Initial training relating to the survey being carried out in Bolikhamxay province was conducted in May 2002. This training was not a particular course as such, but rather a detailed look at/discussion of the research objectives of the Bolikhamxay field study and how we would achieve these objectives. Issues covered included: (i) research plan and expected outputs (ii) information collection and recording (iii) a detailed breakdown of the monthly survey forms was carried out so that provincial and district staff responsible for the survey fully understood what information is collected and (iv) sample collection and submission.

Year 5

Implementation of CSF preventative and control strategies in village production systems combined with longitudinal studies to measure their effectiveness and sustainability
(a) Swine production and health survey in selected villages of Bolikhamxay province
Baseline surveys were conducted in May 2002 in 8 villages, 4 villages in each of 2 districts, namely Bolikhan and Pakading, to gather information on village demography, pig management, production and health.
On a monthly basis pig production and health data are collected from each village
As yet no major losses of stock have occurred as a result of CSF or other infectious/contagious agents during the course of the survey. However, a laboratory confirmed outbreak of CSF occurred in Nampa village in Bolikhan district approximately 2 months prior to the commencement of the survey, killing more than 100 pigs.
The average birthing rates to date in Bolikhan and Pakading districts are 6 and 5.7 piglets/sow respectively
Approximately 58% of total pigs outgoing from farm (deaths, sales and home consumption) are piglets in the age group 0 '€

Year 6

Implementation of CSF preventative and control strategies in village production systems combined with longitudinal studies to measure their effectiveness and sustainability
(a) Swine production and health survey in selected villages of Bolikhamxay province
Baseline surveys were conducted in May 2002 in 8 villages, 4 villages in each of 2 districts, namely Bolikhan and Pakading, to gather information on village demography, pig management, production and health.
On a monthly basis pig production and health data are collected from each village
As yet no major losses of stock have occurred as a result of CSF or other infectious/contagious agents during the course of the survey. However, a laboratory confirmed outbreak of CSF occurred in Nampa village in Bolikhan district approximately 2 months prior to the commencement of the survey, killing more than 100 pigs.
The average birthing rates to date in Bolikhan and Pakading districts are 6 and 5.7 piglets/sow respectively
Approximately 58% of total pigs outgoing from farm (deaths, sales and home consumption) are piglets in the age group 0 - 3 months. The break down of information regarding out-of-village sales is on going.
The price per kilo live weight offered to farmers was 6000 kip. Considering the majority of sales are in the age bracket 0 - 3 months, farmers are receiving only a few dollars for their stock. There are a number of reasons why this could be the case, (i) market driven (consumers prefer young pig meat), (ii) fear of disease, (iii) not enough feed to hold piglets longer or (iv) a combination of the first 3 points.
Approximately 9% of the production system output is lost to the farmers through death of stock, of this 9%, approximately 79% of deaths occur in stock 0 - 3 months of age. Further data analysis is necessary.
Training has been carried out to allow this survey to be conducted.

(b) Maternal transfer of local CSF vaccine derived antibodies in controlled lab based experiments and under less controlled village-based experiments
This study is in progress. 4 laboratory-based pigs have been mated and are pregnant. The first piglets are due to be born in late March 2003. Flooding of the laboratory pens in July - September 2002 resulted in an unavoidable delay in commencing this experiment. It is due to be completed within the next three months
Contracts are currently being negotiated with farmers in Nampa village in Bolikhan district to carry out maternal antibody transfer experiments in 4 pregnant sows in the villages. It is expected that piglets will be born in April 2003.

Complete investigations of the epidemiology of recent FMD outbreaks in the north of the Lao PDR
Structured retrospective epidemiological investigations of FMD outbreaks in Luang Namtha and Xieng Khouang provinces of the Lao PDR were carried out in September and October 2002. Outbreaks of FMD had not been previously observed in either of these two provinces. In both locations type O caused the outbreaks that occurred in nave herds, as farmers in outbreak areas had not previously recognised FMD in their stock. Outbreaks were found to be the result of the introduction of infected stock (Xieng Khouang) and possibly through the importation of contaminated meat (Luang Namtha). The principle mode of spread of the virus once established in both provinces was the within and between village contact of animals and the localised movement and trade of animals. The latter seemed to be exacerbated by the common practice of farmers selling their animals when they become sick and "middleman" traders taking advantage of farmers' willingness to sell their animals at a lower price. The pattern of sale and the movement of animals for this purpose were quite varied between Luang Namtha and Xieng Khouang. The frequency of trade in each province was not determined, however this practice was far more obvious in Xieng Khouang where trade was recognised by farmers as a risk high factor (particularly Nong Het district) and more commonly transcended the bounds of local trade, with animals moving to the Vietnamese border from Paek and Kham districts. The outbreak in Luang Namtha resolved after a few months through animal movement restriction, vaccination and the remoteness and difficulty of the terrain. The outbreak in Xieng Khouang has not yet been resolved (as of Jan 2003 outbreaks are still occurring).
Further investigations and surveillance in Xieng Khouang are planned for May 2003.
During the investigation of FMD in Luang Namtha, it became apparent that a substantial number of buffaloes are transported down the Mekong River from Xoploy in Long district (LN province) to Huay Xay in Bokeo province. These animals are not traded legally and pose a significant threat to Lao, Myanmar and Thailand buffalo, cattle and pig populations. It is planned to carry out an investigation to determine the actual number of animals being traded each month on this route and carry out serological surveillance.

Evaluation of modified serological tests for FMD
Initial tests have been completed. A problem with the rabbit and guinea pig antibodies used in the ELISA were suspected. This has been confirmed and it is expected that this technology will be successfully transferred by June 2003.

Conduct training and refresher courses for district animal health staff involved in the field investigations of CSF vaccination and refresh the diagnostic sample submission network (DSSN) where appropriate
Initial training relating to the survey being carried out in Bolikhamxay province was conducted in May 2002. This training was not a particular course as such, but rather a detailed look at/discussion of the research objectives of the Bolikhamxay field study and how we would achieve these objectives. Issues covered included: (i) research plan and expected outputs (ii) information collection and recording (iii) a detailed breakdown of the monthly survey forms was carried out so that provincial and district staff responsible for the survey fully understood what information is collected and (iv) sample collection and submission.

Project Outcomes

The project established a functional field investigation network and an operational virology laboratory in Lao PDR, both of which were major benefits to the country. Three ELISA tests for FMDV and two for CSFV were established and significant progress was made in establishing the epidemiological characteristics of FMD and CSF. Two out of three pig breed susceptibility studies were completed. Molecular epidemiological studies were commenced in Lao PDR and at AAHL/CSIRO in Australia.
The laboratory became the main player in an attempt to control an outbreak of FMD in southern Laos. At the request of the Laotian Government, AusAID made funds available, through ACIAR, for the purchase of half a million doses of FMD vaccine. The researchers diagnosed the particular strain of the FMD virus responsible for the outbreak, identified and ordered the appropriate vaccine, and commenced the development of a control strategy.
In China all five ELISA tests for FMD and CSF were established. Of these three are in routine use, complementing existing tests. The lasting impacts of the project were considered to be the increased capacity for diagnosis of FMD and CSF in Lao and the strengthening of the diagnostic institutional structure of the Yunnan Tropical and Sub Tropical Animal Virus Laboratory (YTSAVDL) at Kunming.
The review team believed that the success of the project was due to the strong commitment of all project scientists and in particular the AVI scientist. The project had strong support from all levels of government in all countries as well as developing strong linkages to the International Atomic Energy Agency (IAEA), FAO and the South East Asian FMD compaign of the Office International des Epizooties (OIE). Regular meetings between Laos and China (Kunming) on their common border have led to the establishment by Kunming of a border post to monitor live animal movements and test for specific diseases.

Australia benefited from the development of additional diagnostic tests to FMD and CSF. Some work still to be completed was transferred to a new ACIAR medium project, AS1/2003/001 Management of CSF and FMD at the village level in Lao PDR.

Location

There are no project locations defined for this project.